Simultaneous assessment of mechanical and electrical function in Langendorff-perfused ex-vivo mouse hearts.

ABSTRACT:

Background

The Langendorff-perfused ex-vivo isolated heart model has been extensively used to study cardiac function for many years. However, electrical and mechanical function are often studied separately-despite growing proof of a complex electro-mechanical interaction in cardiac physiology and pathology. Therefore, we developed an isolated mouse heart perfusion system that allows simultaneous recording of electrical and mechanical function.

Methods

Isolated mouse hearts were mounted on a Langendorff setup and electrical function was assessed via a pseudo-ECG and an octapolar catheter inserted in the right atrium and ventricle. Mechanical function was simultaneously assessed via a balloon inserted into the left ventricle coupled with pressure determination. Hearts w

SUBMITTER: Louradour J 

PROVIDER: S-EPMC10663365 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature