A novel tailed primer nucleic acid test for detection of HPV 16, 18 and 45 DNA at the point of care.

ABSTRACT: Cervical cancer is a leading cause of death for women in low-resource settings despite being preventable through human papillomavirus (HPV) vaccination, early detection, and treatment of precancerous lesions. The World Health Organization recommends high-risk HPV (hrHPV) as the preferred cervical cancer screening strategy, which is difficult to implement in low-resource settings due to high costs, reliance on centralized laboratory infrastructure, and long sample-to-answer times. To help meet the need for rapid, low-cost, and decentralized cervical cancer screening, we developed tailed primer isothermal amplification and lateral flow detection assays for HPV16, HPV18, and HPV45 DNA. We translated these assays into a self-contained cartridge to achieve multiplexed detection of three hrHPV g

SUBMITTER: Chang MM 

PROVIDER: S-EPMC10663460 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature