Temporal patterns of gene expression in response to inoculation with a virulent Anaplasma phagocytophilum strain in sheep.

ABSTRACT: The aim of this study was to characterize the gene expression of host immune- and cellular responses to a Norwegian virulent strain of Anaplasma phagocytophilum, the cause of tick-borne fever in sheep. Ten sheep were intravenously inoculated with a live virulent strain of A. phagocytophilum. Clinical-, observational-, hematological data as well as bacterial load, flow cytometric cell count data from peripheral blood mononuclear cells and host's gene expression post infection was analysed. The transcriptomic data were assessed for pre-set time points over the course of 22 days following the inoculation. Briefly, all inoculated sheep responded with clinical signs of infection 3 days post inoculation and onwards with maximum bacterial load observed on day 6, consistent with tick-borne fever.

SUBMITTER: Eskeland S 

PROVIDER: S-EPMC10663591 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature