Isolation and characterization of Trichoderma harzianum L-methioninase with promising a powerful anticancer.

ABSTRACT: Bioactive components derived from medicinal herbs have recently acquired popularity due to their efficacy in treating various ailments, including cancer and infectious diseases. In this study, the anticancer enzyme, L-methioninase isolated from medicinal plants endophytic fungi, then evaluated its promising therapeutic agents against different types of human cancers. L methionine was purified using column chromatography with the stationary phase of Sephadex G-200 with 6.6-fold purification, which increased the specific activity of 71.3 U/mg of protein with a recovery rate of 48.2 %. On the SDS-PAGE chromatogram, the apparent molecular mass of the isolated enzyme was 48 kDa, and its highest activity was observed at pH 8 and 35 °C. The enzyme was catalytically stable within the pH ran

SUBMITTER: Ashkan MF 

PROVIDER: S-EPMC10663931 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature