Differential expression pattern of Bcl-2 family members in B and T cells in systemic lupus erythematosus and rheumatoid arthritis.

ABSTRACT:

Objective

This study aimed to evaluate the expression level of anti-apoptotic Bcl-2 family proteins in B and T cells in patients with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) in relation to disease activity and the effect of various Bcl-2 family inhibitors (BH3 mimetics) as potential treatment.

Methods

We included 14 SLE patients, 12 RA patients, and 13 healthy controls to study anti-apoptotic Bcl-2, Bcl-XL, and Mcl-1 expression and cell survival in different B and T cell subsets using stimulation assays and intracellular flow cytometry. Effect of various BH3 mimetics was assessed by cell viability analyses.

Results

In SLE, significant differences in Bcl-2 family members were confined to the B cell compartment with decreased induction of Bcl-XL

SUBMITTER: Kielbassa K 

PROVIDER: S-EPMC10664305 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature