CDK5 promotes apoptosis and attenuates chemoresistance in gastric cancer via E2F1 signaling.

ABSTRACT:

Background

Chemoresistance is a major clinical challenge that leads to tumor metastasis and poor clinical outcome. The mechanisms underlying gastric cancer resistance to chemotherapy are still unclear.

Methods

We conducted bioinformatics analyses of publicly available patient datasets to establish an apoptotic phenotype and determine the key pathways and clinical significance. In vitro cell models, in vivo mouse models, and numerous molecular assays, including western blotting, qRT-PCR, immunohistochemical staining, and coimmunoprecipitation assays were used to clarify the role of factors related to apoptosis in gastric cancer in this study. Differences between datasets were analyzed using the Student's t-test and two-way ANOVA; survival rates were estimated based on Kaplan-

SUBMITTER: Cao LL 

PROVIDER: S-EPMC10664659 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature