Rapidly determining the 3D structure of proteins by surface-enhanced Raman spectroscopy.

ABSTRACT: Despite great advances in protein structure analysis, label-free and ultrasensitive methods to obtain the natural and dynamic three-dimensional (3D) structures are still urgently needed. Surface-enhanced Raman spectroscopy (SERS) can be a good candidate, whereas the complexity originated from the interactions between the protein and the gradient surface electric field makes it extremely challenging to determine the protein structure. Here, we propose a deciphering strategy for accurate determination of 3D protein structure from experimental SERS spectra in seconds by simply summing SERS spectra of isolated amino acids in electric fields of different strength with their orientations in protein. The 3D protein structure can be reconstructed by comparing the experimental spectra obtained in a

SUBMITTER: Ma H 

PROVIDER: S-EPMC10665000 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature