Establishment and identification of an animal model of Hirschsprung disease in suckling mice.

ABSTRACT:

Background

Hirschsprung disease (HSCR) is a congenital intestinal malformation. Previous HSCR animal model needs invasive operation on adult animal. The aim of this study is to establish an early-onset animal model which is consistent with the clinical manifestation of HSCR patients.

Methods

The neonatal mice were randomly divided into the benzalkonium chloride (BAC) group, treated with BAC via enema, and the control group, treated with saline. Weight changes, excretion time of carmine, CT scan, hematoxylin-eosin staining and immunofluorescence staining were used to evaluate the effect of the model. Differentially expressed genes (DEGs) in the HSCR mice were analyzed by using DAVID 6.8 database and compared with DEGs from HSCR patients.

Results

The weight of mice was

SUBMITTER: Lan C 

PROVIDER: S-EPMC10665188 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature