Embryo-scale reverse genetics at single-cell resolution.

ABSTRACT: The maturation of single-cell transcriptomic technologies has facilitated the generation of comprehensive cellular atlases from whole embryos^1-4. A majority of these data, however, has been collected from wild-type embryos without an appreciation for the latent variation that is present in development. Here we present the 'zebrafish single-cell atlas of perturbed embryos': single-cell transcriptomic data from 1,812 individually resolved developing zebrafish embryos, encompassing 19 timepoints, 23 genetic perturbations and a total of 3.2 million cells. The high degree of replication in our study (eight or more embryos per condition) enables us to estimate the variance in cell type abundance organism-wide and to detect perturbation-dependent deviance in cell type composition relat

SUBMITTER: Saunders LM 

PROVIDER: S-EPMC10665197 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature