Fluorescent molecular rotors as versatile in situ sensors for protein quantitation.

ABSTRACT: Accurate protein quantitation is essential for many cellular mechanistic studies. Existing technology relies on extrinsic sample evaluation that requires significant volumes of sample as well as addition of assay-specific reagents and importantly, is a terminal analysis. This study exploits the unique chemical features of a fluorescent molecular rotor that fluctuates between twisted-to-untwisted states, with a subsequent intensity increase in fluorescence depending on environmental conditions (e.g., viscosity). Here we report the development of a rapid, sensitive in situ protein quantitation method using ARCAM-1, a representative fluorescent molecular rotor that can be employed in both non-terminal and terminal assays.

SUBMITTER: Daus K 

PROVIDER: S-EPMC10665405 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature