Single-cell epigenomics and spatiotemporal transcriptomics reveal human cerebellar development.

ABSTRACT: Human cerebellar development is orchestrated by molecular regulatory networks to achieve cytoarchitecture and coordinate motor and cognitive functions. Here, we combined single-cell transcriptomics, spatial transcriptomics and single cell chromatin accessibility states to systematically depict an integrative spatiotemporal landscape of human fetal cerebellar development. We revealed that combinations of transcription factors and cis-regulatory elements (CREs) play roles in governing progenitor differentiation and cell fate determination along trajectories in a hierarchical manner, providing a gene expression regulatory map of cell fate and spatial information for these cells. We also illustrated that granule cells located in different regions of the cerebellar cortex showed distinct molecu

SUBMITTER: Zhong S 

PROVIDER: S-EPMC10665552 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature