HPLC for simultaneous quantification of free mannose and glucose concentrations in serum: use in detection of ovarian cancer.

ABSTRACT: Background: Abnormal levels of monosaccharides in blood have been linked to tumorigenesis. In this study, a novel high-performance liquid chromatography (HPLC) method was established for the simultaneous determination of free mannose and glucose in the serum. Methods: The serum was directly derivatized by 1-phenyl-3-methyl-5-pyrazolone under alkaline conditions using L-rhamnose as an internal standard. The chromatographic separation was then performed on a Poroshell EC-C₁₈ chromatographic column (4.6 × 100 mm, particle size 2.7 μm, Agilent) with gradient elution using NH₄Ac-HAc and acetonitrile as the mobile phases. The method was thereafter validated according to international guidelines. The serum samples obtained from 200 healthy individuals and 200 o

SUBMITTER: Chen Y 

PROVIDER: S-EPMC10665576 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature