Improving thymus implantation for congenital athymia with interleukin-7.

ABSTRACT:

Objectives

Thymus implantation is a recently FDA-approved therapy for congenital athymia. Patients receiving thymus implantation develop a functional but incomplete T cell compartment. Our objective was to develop a mouse model to study clinical thymus implantation in congenital athymia and to optimise implantation procedures to maximise T cell education and expansion of naïve T cells.

Methods

Using Foxn1 ^nu athymic mice as recipients, we tested MHC-matched and -mismatched donor thymi that were implanted as fresh tissue or cultured to remove donor T cells. We first implanted thymus under the kidney capsule and then optimised intramuscular implantation. Using competitive adoptive transfer assays, we investigated whether the failure of newly developed T cells

SUBMITTER: Min H 

PROVIDER: S-EPMC10665642 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature