Neuropeptide stimulation of physiological and immunological responses in precision-cut lung slices.

ABSTRACT: Organotypic lung slices, sometimes known as precision-cut lung slices (PCLS), provide an environment in which numerous cell types and interactions can be maintained outside the body (ex vivo). PCLS were maintained ex vivo for up to a week and demonstrated health via the presence of neurons, maintenance of tissue morphology, synthesis of mucopolysaccharides, and minimal cell death. Multiple phenotypes of neuronal fibers were present in lung slices with varied size, caliber, and neurotransmitter immunoreactivity. Of the neuropeptides present in fibers, calcitonin gene-related peptide (CGRP) was the most prevalent. Exposing PCLS to recombinant CGRP resulted in the proliferation and dispersion of CD19⁺ B cells in slices taken selectively from females. The number of granules containi

SUBMITTER: Patlin B 

PROVIDER: S-EPMC10665790 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature