The transcriptional regulator EarA and intergenic terminator sequences modulate archaellation in Pyrococcus furiosus.

ABSTRACT: The regulation of archaellation, the formation of archaeal-specific cell appendages called archaella, is crucial for the motility, adhesion, and survival of archaeal organisms. Although the heavily archaellated and highly motile Pyrococcus furiosus is a key model organism for understanding the production and function of archaella in Euryarchaea, the transcriptional regulation of archaellum assembly is so far unknown. Here we show that the transcription factor EarA is the master regulator of the archaellum (arl) operon transcription, which is further modulated by intergenic transcription termination signals. EarA deletion or overexpression strains demonstrate that EarA is essential for archaellation in P. furiosus and governs the degree of archaellation. Providin

SUBMITTER: Stockl R 

PROVIDER: S-EPMC10665913 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature