Exploiting Purine as an Internal Standard for SERS Quantification of Purine Derivative Molecules Released by Bacteria.

ABSTRACT: Surface-enhanced Raman scattering (SERS) is a highly sensitive technique used in diverse biomedical applications including rapid antibiotic susceptibility testing (AST). However, signal fluctuation in SERS, particularly the widespread of signals measured from different batches of SERS substrates, compromises its reliability and introduces potential errors in SERS-AST. In this study, we investigate the use of purine as an internal standard (IS) to recalibrate SERS signals and quantify the concentrations of two important purine derivatives, adenine and hypoxanthine, which are the most important biomarkers used in SERS-AST. Our findings demonstrate that purine IS effectively mitigates SERS signal fluctuations and enables accurate prediction of adenine and hypoxanthine concentrations across a

SUBMITTER: Cheng HW 

PROVIDER: S-EPMC10666080 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature