Optical Screening and Classification of Drug Binding to Proteins in Human Blood Serum.

ABSTRACT: Protein-drug interactions in the human bloodstream are important factors in applications ranging from drug design, where protein binding influences efficacy and dose delivery, to biomedical diagnostics, where rapid, quantitative measurements could guide optimized treatment regimes. Current measurement approaches use multistep assays, which probe the protein-bound drug fraction indirectly and do not provide fundamental structural or dynamic information about the in vivo protein-drug interaction. We demonstrate that ultrafast 2D-IR spectroscopy can overcome these issues by providing a direct, label-free optical measurement of protein-drug binding in blood serum samples. Four commonly prescribed drugs, known to bind to human serum albumin (HSA), were added to pooled human serum at phys

SUBMITTER: Rutherford SH 

PROVIDER: S-EPMC10666086 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature