CyGate Provides a Robust Solution for Automatic Gating of Single Cell Cytometry Data.

ABSTRACT: To gain a better understanding of the complex human immune system, it is necessary to measure and interpret numerous cellular protein expressions at the single cell level. Mass cytometry is a relatively new technology that offers unprecedented information about the protein expression of a single cell. Conversely, the analysis of high-dimensional and multiparametric mass cytometric data sets presents a new computational challenge. For instance, conventional "manual gating" analysis was inefficient and unreliable for multiparametric phenotyping of the heterogeneous immune cellular system; consequently, automated methods have been developed to address the high dimensionality of mass cytometry data and enhance the reproducibility of the analysis. Here, we present CyGate, a semiautomated method

SUBMITTER: Na S 

PROVIDER: S-EPMC10666088 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature