Pitfalls in RNA Modification Quantification Using Nucleoside Mass Spectrometry.

ABSTRACT: ConspectusIn recent years, there has been a high interest in researching RNA modifications, as they are involved in many cellular processes and in human diseases. A substantial set of enzymes within the cell, called RNA writers, place RNA modifications selectively and site-specifically. Another set of enzymes, called readers, recognize these modifications which guide the fate of the modified RNA. Although RNA is a transient molecule and RNA modification could be removed by RNA degradation, a subclass of enzymes, called RNA erasers, remove RNA modifications selectively and site-specifically to alter the characteristics of the RNA. The detection of RNA modifications can be done by various methods including second and next generation sequencing but also mass spectrometry. An approach capable

SUBMITTER: Ammann G 

PROVIDER: S-EPMC10666278 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature