Disrupted cardiac fibroblast BCAA catabolism contributes to diabetic cardiomyopathy via a periostin/NAP1L2/SIRT3 axis.

ABSTRACT:

Background

Periostin is an extracellular matrix protein that plays a critical role in cell fate determination and tissue remodeling, but the underlying role and mechanism of periostin in diabetic cardiomyopathy (DCM) are far from clear. Thus, we aimed to clarify the mechanistic participation of periostin in DCM.

Methods

The expression of periostin was examined in DCM patients, diabetic mice and high glucose (HG)-exposed cardiac fibroblasts (CF). Gain- and loss-of-function experiments assessed the potential role of periostin in DCM pathogenesis. RNA sequencing was used to investigate the underlying mechanisms of periostin in DCM.

Results

A mouse cytokine antibody array showed that the protein expression of periostin was most significantly upregulated in diabetic mouse

SUBMITTER: Lu QB 

PROVIDER: S-EPMC10666354 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature