Optimized peptide nanofibrils as efficient transduction enhancers for in vitro and ex vivo gene transfer.

ABSTRACT: Chimeric antigen receptor (CAR)-T cell therapy is a groundbreaking immunotherapy for cancer. However, the intricate and costly manufacturing process remains a hurdle. Improving the transduction rate is a potential avenue to cut down costs and boost therapeutic efficiency. Peptide nanofibrils (PNFs) serve as one such class of transduction enhancers. PNFs bind to negatively charged virions, facilitating their active engagement by cellular protrusions, which enhances virion attachment to cells, leading to increased cellular entry and gene transfer rates. While first-generation PNFs had issues with aggregate formation and potential immunogenicity, our study utilized in silico screening to identify short, endogenous, and non-immunogenic peptides capable of enhancing transduction. This le

SUBMITTER: Rauch-Wirth L 

PROVIDER: S-EPMC10666768 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature