Exploring Bacillus amyloliquefaciens strain OM81 for the production of polyhydroxyalkanoate (PHA) bioplastic using olive mill wastewater.

ABSTRACT: In this study, bacterial strains isolated from olive oil mill wastewater assigned to Bacillus (n = 4) and Klebsiella (n = 1) genera, were evaluated for their ability to accumulate intracellular PHA granules using Sudan Black staining. A maximum PHA production of 0.14 g/L (i.e., 30.2% wt./wt. in dry biomass) was observed in Bacillus amyloliquefaciens strain OM81 after 72 h of incubation in the presence of 2% glucose (synthetic medium). To reduce bioplastic production costs and recover a polluting product, olive mill wastewater was tested as a carbon source. In this context, the maximum growth (1.45 g/L) was observed in the presence of 50% olive mill wastewater. After extracting the biopolymers with chloroform, quantitative and qualitative analyses were con

SUBMITTER: Bacha S 

PROVIDER: S-EPMC10667205 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature