Epidermal-dermal coupled spheroids are important for tissue pattern regeneration in reconstituted skin explant cultures.

ABSTRACT: Tissue patterning is critical for the development and regeneration of organs. To advance the use of engineered reconstituted skin organs, we study cardinal features important for tissue patterning and hair regeneration. We find they spontaneously form spheroid configurations, with polarized epidermal cells coupled with dermal cells through a newly formed basement membrane. Functionally, the spheroid becomes competent morphogenetic units (CMU) that promote regeneration of tissue patterns. The emergence of new cell types and molecular interactions during CMU formation was analyzed using scRNA-sequencing. Surprisingly, in newborn skin explants, IFNr signaling can induce apical-basal polarity in epidermal cell aggregates. Dermal-Tgfb induces basement membrane formation. Meanwhile, VEGF signali

SUBMITTER: Lei M 

PROVIDER: S-EPMC10667216 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature