Three-dimensional genome architecture coordinates key regulators of lineage specification in mammary epithelial cells.

ABSTRACT: Although lineage-specific genes have been identified in the mammary gland, little is known about the contribution of the 3D genome organization to gene regulation in the epithelium. Here, we describe the chromatin landscape of the three major epithelial subsets through integration of long- and short-range chromatin interactions, accessibility, histone modifications, and gene expression. While basal genes display exquisite lineage specificity via distal enhancers, luminal-specific genes show widespread promoter priming in basal cells. Cell specificity in luminal progenitors is largely mediated through extensive chromatin interactions with super-enhancers in gene-body regions in addition to interactions with polycomb silencer elements. Moreover, lineage-specific transcription factors appear

SUBMITTER: Milevskiy MJG 

PROVIDER: S-EPMC10667557 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature