Differential labelling of human sub-cellular compartments with fluorescent dye esters and expansion microscopy.

ABSTRACT: Amine-reactive esters of aromatic fluorescent dyes are emerging as imaging probes for nondescript staining of cellular and tissue architectures. We characterised the staining patterns of 14 fluorescent dye ester species with varying physical and spectral properties in the broadly studied human HeLa cell line. When combined with the super-resolution technique expansion microscopy (ExM) involving swellable acrylamide hydrogels, fluorescent esters reveal nanoscale features including cytoplasmic membrane-bound compartments and nucleolar densities. We observe differential labelling patterns linked to the biochemical properties of the conjugated dye. Alterations in staining density and compartment specificity were seen depending on the timepoint of application in the ExM protocol. Additional com

SUBMITTER: Sheard TMD 

PROVIDER: S-EPMC10667587 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature