Dataset Information

Detection, fate and transport of the biohazardous agent Toxoplasma gondii in soil water systems: Influence of soil physicochemical properties, water chemistry and surfactant.

ABSTRACT: A series of laboratory experiments were conducted to study the fate and transport of Toxoplasma gondii oocysts in soils as a function of soil physicochemical properties and soil water chemistry properties. Soil columns were homogeneously packed with loamy sand soils (Lewiston and Greenson series) and sandy loam soils (Sparta and Gilford series), and subject to hydrologic conditions characterized by the absence and presence of an anionic surfactant-Aerosol 22 in the artificial rainfall. Quantitative polymerase chain reaction (qPCR) was utilized for the detection and enumeration of oocysts in soil leachates to evaluate their breakthrough and in soil matrices to examine their spatial distribution. Differences in the rate and extent of transport of oocysts were observed as a function of physical and chemical parameters tested. The breakthrough of oocysts was observed for all the soils irrespective of the presence of surfactant. However, in the absence of surfactant, the predominant fate of oocysts in soils subject to simulated rainfall was their retention in the soil profile. The presence of surfactant induced a change in the fate of oocysts in these soils exposed to rainfall simulation as the predominant fate of oocysts was found to be in the soil leachates.

SUBMITTER: Kinsey EN

PROVIDER: S-EPMC10667666 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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Publications

Detection, fate and transport of the biohazardous agent Toxoplasma gondii in soil water systems: Influence of soil physicochemical properties, water chemistry and surfactant.

Kinsey Erin N EN Korte Caroline C Gouasmia Sohib S L'Ollivier Coralie C Dubey Jitender P JP Dumètre Aurélien A Darnault Christophe J G CJG

Environmental microbiology reports 20230922 6

A series of laboratory experiments were conducted to study the fate and transport of Toxoplasma gondii oocysts in soils as a function of soil physicochemical properties and soil water chemistry properties. Soil columns were homogeneously packed with loamy sand soils (Lewiston and Greenson series) and sandy loam soils (Sparta and Gilford series), and subject to hydrologic conditions characterized by the absence and presence of an anionic surfactant-Aerosol 22 in the artificial rainfall. Quantitat ...[more]

PMID: 37740249

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Project description:BackgroundPredicting the spatial distribution of pathogens with an environmental stage is challenging because of the difficulty to detect them in environmental samples. Among these pathogens, the parasite Toxoplasma gondii is the causative agent of the zoonosis toxoplasmosis, which is responsible for public health issues. Oocysts of T. gondii are excreted by infected cats in the environment, where they may survive and remain infectious for intermediate hosts, specifically rodents, during months to years. The landscape structure that determines the density and distribution of cats may thus impact the spatial distribution of T. gondii. In this study, we investigated the influences of rural settings on the spatial distribution of oocysts in the soil.MethodWe developed a spatially explicit agent based model to study how landscape structures impact on the spatial distribution of T. gondii prevalence in its rodent intermediate host as well as contamination in the environment. The rural landscape was characterized by the location of farm buildings, which provide shelters and resources for the cats. Specifically, we considered two configurations of farm buildings, i.e. inside and outside a village. Simulations of the first setting, with farm buildings inside the village, were validated using data from previous field studies. Then, simulation results of the two settings were compared to investigate the influences of the farm locations.ResultsModel predictions showed a steeper relationship between distance to the nearest farm and infection levels when farm buildings, and thus cats, were concentrated in the same area than when the farms were spread over the area. The relationship between distance to the village center and level of environmental contamination also differed between settings with a potential increased risk for inhabitants when farms are located inside the village. Maps of the risk of soil contaminated with oocysts were also derived from the model.ConclusionThe agent-based model provides a useful tool to assess the risk of contamination by T. gondii oocysts at a local scale and determine the most at risk areas. Moreover it provides a basis to investigate the spatial dynamics of pathogens with an environmental stage.

Dataset Information

Detection, fate and transport of the biohazardous agent Toxoplasma gondii in soil water systems: Influence of soil physicochemical properties, water chemistry and surfactant.

Publications

Detection, fate and transport of the biohazardous agent Toxoplasma gondii in soil water systems: Influence of soil physicochemical properties, water chemistry and surfactant.

Similar Datasets

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