Physiological Replication of the Human Glomerulus Using a Triple Culture Microphysiological System.

ABSTRACT: The function of the glomerulus depends on the complex cell-cell/matrix interactions and replication of this in vitro would aid biological understanding in both health and disease. Previous models do not fully reflect all cell types and interactions present as they overlook mesangial cells within their 3D matrix. Herein, the development of a microphysiological system that contains all resident renal cell types in an anatomically relevant manner is presented. A detailed transcriptomic analysis of the contributing biology of each cell type, as well as functionally appropriate albumin retention in the system, is demonstrated. The important role of mesangial cells is shown in promoting the health and maturity of the other cell types. Additionally, a comparison of the incremental advances that e

SUBMITTER: Pajoumshariati R 

PROVIDER: S-EPMC10667800 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature