Actomyosin Activity and Piezo1 Activity Synergistically Drive Urinary System Fibroblast Activation.

ABSTRACT: Mechanical cues play a crucial role in activating myofibroblasts from quiescent fibroblasts during fibrosis, and the stiffness of the extracellular matrix is of significant importance in this process. While intracellular force mediated by myosin II and calcium influx regulated by Piezo1 are the primary mechanisms by which cells sense and respond to mechanical forces, their intercellular mechanical interaction remains to be elucidated. Here, hydrogels with tunable substrate are used to systematically investigate the crosstalk of myosin II and Piezo1 in fibroblast to myofibroblast transition (FMT). The findings reveal that the two distinct signaling pathways are integrated to convert mechanical stiffness signals into biochemical signals during bladder-specific FMT. Moreover, it is demonstrat

SUBMITTER: Chen G 

PROVIDER: S-EPMC10667826 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature