Double Digital Assay for Single Extracellular Vesicle and Single Molecule Detection.

ABSTRACT: Extracellular vesicles (EVs) have emerged as a promising source of biomarkers for disease diagnosis. However, current diagnostic methods for EVs present formidable challenges, given the low expression levels of biomarkers carried by EV samples, as well as their complex physical and biological properties. Herein, a highly sensitive double digital assay is developed that allows for the absolute quantification of individual molecules from a single EV. Because the relative abundance of proteins is low for a single EV, tyramide signal amplification (TSA) is integrated to increase the fluorescent signal readout for evaluation. With the integrative microfluidic technology, the technology's ability to compartmentalize single EVs is successfully demonstrated, proving the technology's digital partit

SUBMITTER: Reynolds DE 

PROVIDER: S-EPMC10667851 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature