Sec71 separates Golgi stacks in Drosophila S2 cells.

ABSTRACT: Golgi stacks are the basic structural units of the Golgi. Golgi stacks are separated from each other and scattered in the cytoplasm of Drosophila cells. Here, we report that the ARF-GEF inhibitor Brefeldin A (BFA) induces the formation of BFA bodies, which are aggregates of Golgi stacks, trans-Golgi networks and recycling endosomes. Recycling endosomes are located in the centers of BFA bodies, while Golgi stacks surround them on their trans sides. Live imaging of S2 cells revealed that Golgi stacks repeatedly merged and separated on their trans sides, and BFA caused successive merger by inhibiting separation, forming BFA bodies. S2 cells carrying genome-edited BFA-resistant mutant Sec71^M717L did not form BFA bodies at high concentrations of BFA; S2 ce

SUBMITTER: Fujii S 

PROVIDER: S-EPMC10668125 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature