Discrimination between the effects of pulsed electrical stimulation and electrochemically conditioned medium on human osteoblasts.

ABSTRACT:

Background

Electrical stimulation is used for enhanced bone fracture healing. Electrochemical processes occur during the electrical stimulation at the electrodes and influence cellular reactions. Our approach aimed to distinguish between electrochemical and electric field effects on osteoblast-like MG-63 cells. We applied 20 Hz biphasic pulses via platinum electrodes for 2 h. The electrical stimulation of the cell culture medium and subsequent application to cells was compared to directly stimulated cells. The electric field distribution was predicted using a digital twin.

Results

Cyclic voltammetry and electrochemical impedance spectroscopy revealed partial electrolysis at the electrodes, which was confirmed by increased concentrations of hydrogen peroxide in the medium. Wh

SUBMITTER: Bielfeldt M 

PROVIDER: S-EPMC10668359 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature