Role of Relebactam in the Antibiotic Resistance Acquisition in Pseudomonas aeruginosa: In Vitro Study.

ABSTRACT:

Background

Pseudomonas aeruginosa shows resistance to several antibiotics and often develops such resistance during patient treatment.

Objective

Develop an in vitro model, using clinical isolates of P. aeruginosa, to compare the ability of the imipenem and imipenem/relebactam to generate resistant mutants to imipenem and to other antibiotics. Perform a genotypic analysis to detect how the selective pressure changes their genomes.

Methods

The antibiotics resistance was studied by microdilution assays and e-test, and the genotypic study was performed by NGS.

Results

The isolates acquired resistance to imipenem in an average of 6 days, and to imipenem/relebactam in 12 days (p value = 0.004). After 30 days of exposure, 75% of the isolates reach

SUBMITTER: Ventero MP 

PROVIDER: S-EPMC10668777 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature