Interactions of ruthenium(II) polypyridyl complexes with human telomeric DNA.

ABSTRACT: Eight [Ru(bpy)₂L]²⁺ and three [Ru(phen)₂L]²⁺complexes (where bpy = 2,2'-bipyridine and phen = 1,10-phenanthroline are ancillary ligands, and L = a polypyridyl experimental ligand) were investigated for their G-quadruplex binding abilities. Fluorescence resonance energy transfer melting assays were used to screen these complexes for their ability to selectively stabilize human telomeric DNA variant, Tel22. The best G-quadruplex stabilizers were further characterized for their binding properties (binding constant and stoichiometry) using UV-vis, fluorescence spectroscopy, and mass spectrometry. The ligands' ability to alter the structure of Tel22 was determined via circular dichroism and PAGE studies. We identified me₂allox as the experi

SUBMITTER: Tran VT 

PROVIDER: S-EPMC10668861 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature