Dynamics of Methane-Consuming Biomes from Wieliczka Formation: Environmental and Enrichment Studies.

ABSTRACT: The rocks surrounding Wieliczka salt deposits are an extreme, deep subsurface ecosystem that as we studied previously harbors many microorganisms, including methanotrophs. In the presented research bacterial community structure of the Wieliczka Salt Mine was determined as well as the methanotrophic activity of the natural microbiome. Finally, an enrichment culture of methane-consuming methanotrophs was obtained. The research material used in this study consisted of rocks surrounding salt deposits in the Wieliczka Salt Mine. DNA was extracted directly from the pristine rock material, as well as from rocks incubated in an atmosphere containing methane and mineral medium, and from a methanotrophic enrichment culture from this ecosystem. As a result, the study describes the composition of the

SUBMITTER: Goraj W 

PROVIDER: S-EPMC10669130 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature