Lactose Permease Scrambles Phospholipids.

ABSTRACT: Lactose permease (LacY) from Escherichia coli belongs to the major facilitator superfamily. It facilitates the co-transport of β-galactosides, including lactose, into cells by using a proton gradient towards the cell. We now show that LacY is capable of scrambling glycerophospholipids across a membrane. We found that purified LacY reconstituted into liposomes at various protein to lipid ratios catalyzed the rapid translocation of fluorescently labeled and radiolabeled glycerophospholipids across the proteoliposome membrane bilayer. The use of LacY mutant proteins unable to transport lactose revealed that glycerophospholipid scrambling was independent of H⁺/lactose transport activity. Unexpectedly, in a LacY double mutant locked into an occluded conformation glycerophospho

SUBMITTER: Wang L 

PROVIDER: S-EPMC10669175 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature