Characterization of a Human Neuronal Culture System for the Study of Cofilin-Actin Rod Pathology.

ABSTRACT: Cofilactin rod pathology, which can initiate synapse loss, has been extensively studied in rodent neurons, hippocampal slices, and in vivo mouse models of human neurodegenerative diseases such as Alzheimer's disease (AD). In these systems, rod formation induced by disease-associated factors, such as soluble oligomers of Amyloid-β (Aβ) in AD, utilizes a pathway requiring cellular prion protein (PrP^C), NADPH oxidase (NOX), and cytokine/chemokine receptors (CCR5 and/or CXCR4). However, rod pathways have not been systematically assessed in a human neuronal model. Here, we characterize glutamatergic neurons differentiated from human-induced pluripotent stem cells (iPSCs) for the formation of rods in response to activators of the PrP^C-dependent pathway. Optimization of subs

SUBMITTER: Tahtamouni LH 

PROVIDER: S-EPMC10669520 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature