Cellular-Level Analysis of Retinal Blood Vessel Walls Based on Phase Gradient Images.

ABSTRACT: Diseases such as diabetes affect the retinal vasculature and the health of the neural retina, leading to vision problems. We describe here an imaging method and analysis procedure that enables characterization of the retinal vessel walls with cellular-level resolution, potentially providing markers for eye diseases. Adaptive optics scanning laser ophthalmoscopy is used with a modified detection scheme to include four simultaneous offset aperture channels. The magnitude of the phase gradient derived from these offset images is used to visualize the structural characteristics of the vessels. The average standard deviation image provides motion contrast and enables segmentation of the vessel lumen. Segmentation of blood vessel walls provides quantitative measures of geometrical characteristic

SUBMITTER: Mujat M 

PROVIDER: S-EPMC10670340 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature