A New qPCR Assay for the Rapid Diagnosis of Anthonomus grandis Subspecies.

ABSTRACT: Rapid and accurate identification of Anthonomus grandis subspecies is crucial for effective management and eradication. Current diagnostic methods have limitations in terms of time to diagnosis (up to seven days) and can yield ambiguous results. Here, we present the validation of a custom TaqMan SNP Genotyping Assay for the rapid and accurate identification of A. grandis grandis (boll weevil) and A. g. thurberiae (thurberia weevil) subspecies. To validate the assay, we conducted three main experiments: (1) a sensitivity test to determine the DNA concentration range at which the assay performs, (2) a non-target specificity test to ensure no amplification in non-target weevils (false positives), and (3) an accuracy test comparing the results of the new assay to previousl

SUBMITTER: Raszick TJ 

PROVIDER: S-EPMC10671495 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature