WISP1 Is Involved in the Pathogenesis of Kashin-Beck Disease via the Autophagy Pathway.

ABSTRACT:

Objective

Kashin-Beck disease (KBD) is a kind of endemic and chronic osteochondropathy in China. This study aims to explore the functional relevance and potential mechanism of Wnt-inducible signaling pathway protein 1 (WISP1) in the pathogenesis of KBD.

Design

KBD and control cartilage specimens were collected for tissue section observation and primary chondrocyte culture. Firstly, the morphological and histopathological observations were made under a light and electron microscope. Then, the expression levels of WISP1 as well as molecular markers related to the autophagy pathway and extracellular matrix (ECM) synthesis were detected in KBD and control chondrocytes by qRT-PCR, Western blot, and immunohistochemistry. Furthermore, the lentiviral transfection technique was appli

SUBMITTER: Li P 

PROVIDER: S-EPMC10671535 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature