Characterization of an α-Amylase from the Honeybee Chalk Brood Pathogen Ascosphaera apis.

ABSTRACT: The insect pathogenic fungus, Ascosphaera apis, is the causative agent of honeybee chalk brood disease. Amylases are secreted by many plant pathogenic fungi to access host nutrients through the metabolism of starch, and the identification of new amylases can have important biotechnological applications. Production of amylase by A. apis in submerged culture was optimized using the response surface method (RSM). Media composition was modeled using Box-Behnken design (BBD) at three levels of three variables, and the model was experimentally validated to predict amylase activity (R² = 0.9528). Amylase activity was highest (45.28 ± 1.16 U/mL, mean ± SE) in media composed of 46 g/L maltose and1.51 g/L CaCl₂ at a pH of 6.6, where total activity was ~11-f

SUBMITTER: Li J 

PROVIDER: S-EPMC10672707 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature