Membrane Cascade Fractionation of Tomato Leaf Extracts-Towards Bio-Based Crop Protection.

ABSTRACT: Promising initial results from the use of membrane-fractionated extracts of tomato leaf as crop protection agents have recently been reported. This paper provides additional evidence from larger scale experiments that identify an efficient pipeline for the separation of tomato leaf extracts to generate a fraction with significant defence elicitor activity. A UF tubular membrane 150 kDa, with an internal diameter of 5 mm, proved appropriate for initial extract clarification, whereas afterwards a UF 10 kDa and three NF membranes (200-800 Da) in sequence were evaluated for the subsequent fractionation of this tomato extract. The compositions of sugars, proteins and total biophenols were changed in these fractions with respect to the initial extract. The initial extract ratio of sugars: protei

SUBMITTER: Papaioannou EH 

PROVIDER: S-EPMC10673455 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature