Absolute Quantitation of Peptides and Proteins by Coulometric Mass Spectrometry After Derivatization.

ABSTRACT: Peptide/protein quantitation using mass spectrometry (MS) is advantageous due to its high sensitivity. Traditional absolute peptide quantitation methods rely on making calibration curves using peptide standards or isotope-labelled peptide standards, which are expensive and take time to synthesize. A method which can eliminate the need for using standards would be beneficial. Recently, we developed coulometric mass spectrometry (CMS) which can be used to quantify peptides that are oxidizable (e.g., those containing tyrosine or tryptophan), without using peptide standard. The method is based on electrochemical oxidation of peptides followed by MS to measure the oxidation yield. However, it cannot be directly used to quantify peptides without oxidizable residues. To extend this method for qua

SUBMITTER: Fnu PIJ 

PROVIDER: S-EPMC10673616 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature