Evaluating long-read de novo assembly tools for eukaryotic genomes: insights and considerations.

ABSTRACT:

Background

Assembly algorithm choice should be a deliberate, well-justified decision when researchers create genome assemblies for eukaryotic organisms from third-generation sequencing technologies. While third-generation sequencing by Oxford Nanopore Technologies (ONT) and Pacific Biosciences (PacBio) has overcome the disadvantages of short read lengths specific to next-generation sequencing (NGS), third-generation sequencers are known to produce more error-prone reads, thereby generating a new set of challenges for assembly algorithms and pipelines. However, the introduction of HiFi reads, which offer substantially reduced error rates, has provided a promising solution for more accurate assembly outcomes. Since the introduction of third-generation sequencing technologies, many to

SUBMITTER: Cosma BM 

PROVIDER: S-EPMC10673639 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature