High-quality and robust protein quantification in large clinical/pharmaceutical cohorts with IonStar proteomics investigation.

ABSTRACT: Robust, reliable quantification of large sample cohorts is often essential for meaningful clinical or pharmaceutical proteomics investigations, but it is technically challenging. When analyzing very large numbers of samples, isotope labeling approaches may suffer from substantial batch effects, and even with label-free methods, it becomes evident that low-abundance proteins are not reliably measured owing to unsufficient reproducibility for quantification. The MS1-based quantitative proteomics pipeline IonStar was designed to address these challenges. IonStar is a label-free approach that takes advantage of the high sensitivity/selectivity attainable by ultrahigh-resolution (UHR)-MS1 acquisition (e.g., 120-240k full width at half maximum at m/z = 200) which is now widely available on ultra

SUBMITTER: Shen S 

PROVIDER: S-EPMC10673696 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature