Improved production of Bacillus subtilis cholesterol oxidase by optimization of process parameters using response surface methodology.

ABSTRACT:

Background

Cholesterol oxidase has numerous biomedical and industrial applications. In the current study, a new bacterial strain was isolated from sewage and was selected for its high potency for cholesterol degradation (%) and production of high cholesterol oxidase activity (U/OD₆₀₀).

Results

Based on the sequence of 16S rRNA gene, the bacterium was identified as Bacillus subtilis. The fermentation conditions affecting cholesterol degradation (%) and the activity of cholesterol oxidase (U/OD₆₀₀) of B. subtilis were optimized through fractional factorial design (FFD) and response surface methodology (RSM). According to this sequential optimization approach, 80.152% cholesterol degradation was achieved by setting the concentrations of cholesterol, inocul

SUBMITTER: Lotfy WA 

PROVIDER: S-EPMC10673797 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature