Biophysical properties of Na_V1.5 channels from atrial-like and ventricular-like cardiomyocytes derived from human induced pluripotent stem cells.

ABSTRACT: Generating atrial-like cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) is crucial for modeling and treating atrial-related diseases, such as atrial arrythmias including atrial fibrillations. However, it is essential to obtain a comprehensive understanding of the electrophysiological properties of these cells. The objective of the present study was to investigate the molecular, electrical, and biophysical properties of several ion channels, especially Na_V1.5 channels, in atrial hiPSC cardiomyocytes. Atrial cardiomyocytes were obtained by the differentiation of hiPSCs treated with retinoic acid (RA). The quality of the atrial specification was assessed by qPCR, immunocytofluorescence, and western blotting. The electrophysiological properties of action pot

SUBMITTER: Chapotte-Baldacci CA 

PROVIDER: S-EPMC10673932 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature