Comparison of clean catch and bag urine using LC-MS/MS proteomics in infants.

ABSTRACT:

Background

Urinary proteomics identifies the totality of urinary proteins and can therefore help in getting an early and precise diagnosis of various pathological processes in the kidneys. In infants, non-invasive urine collection is most commonly accomplished with a urine bag or clean catch. The influence of those two collection methods on urinary proteomics was assessed in this study.

Methods

Thirty-two urine samples were collected in infants using urine bag and clean catch within 24 h. Nine boys and seven girls with a mean age of 4.3 ± 2.9 months were included (5 × post-pyelonephritis, 10 × non-kidney disease, 1 × chronic kidney disease (CKD)). Liquid chromatography-mass spectrometry (LC-MS/MS) was performed in data-independent acquisition (DIA) mode. Protein identificati

SUBMITTER: Klaus R 

PROVIDER: S-EPMC10673958 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature