Recombinant protein delivery enables modulation of the phototransduction cascade in mouse retina.

ABSTRACT: Inherited retinal dystrophies are often associated with mutations in the genes involved in the phototransduction cascade in photoreceptors, a paradigmatic signaling pathway mediated by G protein-coupled receptors. Photoreceptor viability is strictly dependent on the levels of the second messengers cGMP and Ca²⁺. Here we explored the possibility of modulating the phototransduction cascade in mouse rods using direct or liposome-mediated administration of a recombinant protein crucial for regulating the interplay of the second messengers in photoreceptor outer segments. The effects of administration of the free and liposome-encapsulated human guanylate cyclase-activating protein 1 (GCAP1) were compared in biological systems of increasing complexity (in cyto, ex vivo, and in vivo).

SUBMITTER: Asteriti S 

PROVIDER: S-EPMC10673981 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature