Dataset Information

Diaphragmatic hernia repair porcine model to compare the performance of biodegradable membranes against Gore-Tex^®.

ABSTRACT:

Background

Patch repair of congenital diaphragmatic hernia (CDH) using Gore-Tex^® is associated with infection, adhesions, hernia recurrence, long-term musculoskeletal sequels and poor tissue regeneration. To overcome these limitations, the performance of two novel biodegradable membranes was tested to repair CDH in a growing pig model.

Methods

Twelve male pigs were randomly assigned to 3 different groups of 4 animals each, determined by the type of patch used during thoracoscopic diaphragmatic hernia repair (Gore-Tex^®, polycaprolactone electrospun membrane-PCLem, and decellularized human chorion membrane-dHCM). After 7 weeks, all animals were euthanized, followed by necropsy for diaphragmatic evaluation and histological analysis.

Results

Thoracoscopic defect creation and diaphragmatic repair were performed without any technical difficulty in all groups. However, hernia recurrence rate was 0% in Gore-Tex^®, 50% in PCLem and 100% in dHCM groups. At euthanasia, Gore-Tex^® patches appeared virtually unchanged and covered with a fibrotic capsule, while PCLem and dHCM patches were replaced by either floppy connective tissue or vascularized and floppy regenerated membranous tissue, respectively.

Conclusion

Gore-Tex^® was associated with a higher survival rate and lower recurrence. Nevertheless, the proposed biodegradable membranes were associated with better tissue integration when compared with Gore-Tex^®.

SUBMITTER: Scuglia M

PROVIDER: S-EPMC10673990 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature

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Publications

Diaphragmatic hernia repair porcine model to compare the performance of biodegradable membranes against Gore-Tex<sup>®</sup>.

Scuglia Marianna M Frazão Laura P LP Miranda Alice A Martins Albino A Barbosa-Sequeira Joana J Coimbra Diana D Longatto-Filho Adhemar A Reis Rui L RL Nogueira-Silva Cristina C Neves Nuno M NM Correia-Pinto Jorge J

Pediatric surgery international 20231124 1

<h4>Background</h4>Patch repair of congenital diaphragmatic hernia (CDH) using Gore-Tex<sup>®</sup> is associated with infection, adhesions, hernia recurrence, long-term musculoskeletal sequels and poor tissue regeneration. To overcome these limitations, the performance of two novel biodegradable membranes was tested to repair CDH in a growing pig model.<h4>Methods</h4>Twelve male pigs were randomly assigned to 3 different groups of 4 animals each, determined by the type of patch used during tho ...[more]

PMID: 37999778

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Project description:BackgroundDislocated IOL exchange conventionally involves manipulation within the anterior chamber which risks secondary injury to anterior chamber structures. We describe and evaluate a 4-haptic IOL rescue technique that avoids entering the anterior chamber and thus minimizes post operative inflammation, astigmatism and recovery time relative to conventional IOL explantation and replacement techniques.MethodsRetrospective, non-randomized, interventional study of all patients undergoing 4-haptic IOL rescue performed by two independent vitreoretinal surgeons at a single UK centre over two years.Surgical techniqueA limited peritomy is performed with four 25-gauge scleral ports placed to enable use of two forceps, an infusion and a chandelier. A further four 27-gauge sclerotomies are symmetrically placed on the nasal and temporal sclera at 3 mm from the limbus with a 5 mm vertical separation on either side. A pars plana vitrectomy is performed followed by chandelier illuminated, bimanual cleaning of the dislocated IOL using 27-gauge serrated forceps. Gore-tex sutures are threaded through the IOL islets within the vitreous cavity and externalized through the sclerotomies for scleral re-fixation followed by conjunctival closure.ResultsSeven patients underwent IOL recycling with Gore-Tex suture scleral re-fixation. All procedures were successful in repositioning the IOLs, with all patients satisfied with post-operative outcome. Mean (standard deviation) time to IOL dislocation was 13 (3) years. Median visual acuity significantly improved post-operatively from 0.85 logMAR (Interquartile Range [IQR]: 0.2-2.1) to 0.07 (0.02-0.60) logMAR (p = 0.02). No significant post-operative complications were noted apart from persistent cystoid macular oedema in one patient non-compliant with post-operative treatment.ConclusionsTransscleral refixation using Gore-Tex suture is an effective, safe and practical approach in the management of dislocated 4-piece IOLs.

Dataset Information

Diaphragmatic hernia repair porcine model to compare the performance of biodegradable membranes against Gore-Tex^®.

Background

Methods

Results

Conclusion

Publications

Diaphragmatic hernia repair porcine model to compare the performance of biodegradable membranes against Gore-Tex<sup>®</sup>.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Diaphragmatic hernia repair porcine model to compare the performance of biodegradable membranes against Gore-Tex®.

Background

Methods

Results

Conclusion

Publications

Diaphragmatic hernia repair porcine model to compare the performance of biodegradable membranes against Gore-Tex<sup>®</sup>.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Diaphragmatic hernia repair porcine model to compare the performance of biodegradable membranes against Gore-Tex^®.