Human Pancreatic Islets React to Glucolipotoxicity by Secreting Pyruvate and Citrate.

ABSTRACT: Progressive decline in pancreatic beta-cell function is central to the pathogenesis of type 2 diabetes (T2D). Here, we explore the relationship between the beta cell and its nutritional environment, asking how an excess of energy substrate leads to altered energy production and subsequent insulin secretion. Alterations in intracellular metabolic homeostasis are key markers of islets with T2D, but changes in cellular metabolite exchanges with their environment remain unknown. We answered this question using nuclear magnetic resonance-based quantitative metabolomics and evaluated the consumption or secretion of 31 extracellular metabolites from healthy and T2D human islets. Islets were also cultured under high levels of glucose and/or palmitate to induce gluco-, lipo-, and glucolipotoxicity.

SUBMITTER: Perrier J 

PROVIDER: S-EPMC10674605 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature